Figure 4.
MBD3C Is Redundant with MBD3A and MBD3B in Regulation of Gene Expression
(A and B) MA plots showing log2 (fold change) in gene expression in Mbd3c KO (A, left), Mbd3ab KO (A, right), Mbd3abc KO (B, left), and Mbd3cΔN-ab KO (B, right) ESCs relative to wild-type (WT). Genes shown are misregulated ≥2-fold compared with WT.
(C and D) Venn diagrams showing overlap between misregulated genes in ESCs of indicated genotypes.
(E and F) WDR5 binding (Ang et al., 2011) (E) and MOF binding (Li et al., 2012) (F) averaged over transcription start sites (TSS) of misregulated or unchanged genes in Mbd3abc KO (red) and Mbd3cΔN-ab KO ESCs (purple).
(G) Average WDR5 binding over MBD3-bound (Yildirim et al., 2011), TSS-distal DNase I hypersensitive sites (DHSs) (GSM1014514).