Functional Characterization of BM-MSCs from HDs and AML Patients
(A) Proliferation measured as population doublings of BM-MSC cultures from LR-, IR-, and HR-BM-MSC compared with BM-MSCs from HD. Inset represents patient variability. n = 46 patients (16 LR-, 11 IR-, and 19 HR-AML) and n = 10 healthy donor controls.
(B) Clonogenic capacity of BM-MSCs (number of CFU per 1,000 cells seeded) from HD and LR-, IR- and HR-AML subjects. p < 0.05. Right: representative crystal violet staining of BM-MSC-CFU/colonies from HD- and AML-derived BM-MSCs. n = 40 patients (13 LR-, 10 IR-, and 17 HR-AML) and n = 10 healthy donor controls. Error bars indicate the SEM values of the biological replicates.