Figure 5.

Mechanisms Underlying the Induction of KLF4 by ADR in Regulation of Osteosarcoma Cancer Stemness

(A) Schematic representation of the gene expression profile comparisons of KHOS/NP cells (2-fold difference; blue: ADR versus control; green: KLF4 versus control) and the overlay analysis.

(B) Microarray data of upregulated stem cell/progenitor marker genes in KHOS/NP cells (blue: ADR versus control; green: KLF4 versus control).

(C) Heatmap of genes correlated with cell motility.

(D) Validation of the microarray data of (C) and (B) using qRT-PCR. Selected genes from different functional groups were validated with qRT-PCR. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 versus control. Data are presented as mean ± SD; at least three independent experiments were performed.

(E) Microarray analysis and qRT-PCR detection of SPP1 mRNA expression in KHOS/NP cells. The gene expression levels were validated with qRT-PCR. ^∗∗∗p < 0.001 versus control. qRT-PCR data are presented as mean ± SD of three independent experiments.