Figure 4.
ADP-Evoked [Ca2+]i Transients in Microglia and Macrophages
(A) Left panel shows five example traces of [Ca2+]i transients following ADP application in iPSC-MG loaded with the Ca2+ indicator Fluo-4/AM. Right panel shows time lapse of changes in fluorescence intensity produced by ADP application. Magenta and cyan traces originate from cells indicated by same-colored regions of interest in the right panel. Bars represent duration of ADP or ATP application.
(B and C) Same data as in (A), obtained from primary human microglia (hMG) and hMG-SF correspondingly.
(D) ADP and ATP responses in PB-M(−). Note the absence of significant [Ca2+]i transients in response to ADP.
(E) Statistical analysis for the amplitudes of [Ca2+]i transients. Maximum amplitude of [Ca2+]i transient for each responsive cell is presented as a gray dot in the corresponding category (∗∗∗p < 0.001 by t test).
(F) Percentages of ADP-responsive cells among all different cell types analyzed. Ratios for each cell type indicate the number of responsive cells out of total number of cells analyzed. iPSC-MG data are pooled from three independent experiments.
See also Figure S4.