FIG. 1.

Assays for the determination of MBL, L-ficolin, and H-ficolin concentrations. Dilutions of standard sera with known concentrations of the respective molecules were applied to wells coated with monoclonal antibodies to MBL (A), L-ficolin (B), and H-ficolin (C). Bound proteins were detected by use of relevant biotinylated monoclonal antibodies. The signals are given as counts per second. Error bars indicate standard deviations. (D) Concentrations of H-ficolin, L-ficolin, and MBL in samples from 97 healthy individuals. Note the different y axis for MBL. Each filled circle represents an individual.