TABLE 1.
Summary of TFP loci identified in the B. pseudomallei K96243 genome
| Locus | Gene | Amino acidsa | Subunit/other homologue (organism; GenBank accession no.)b | % Similarity/% identity (amino acidsc) |
|---|---|---|---|---|
| Chromosome 1 | ||||
| TFP1 | BPSL0782 | 207 | Type IVA pilus subunit PilA (Pseudomonas aeruginosa; AAL12242) | 49/32 (217) |
| TFP2 | BPSL1821 | 63 | Type IVB pilus subunit Flp1 (Actinobacillus actinomycetemcomitans; AAN75204) | 42/24 (76) |
| TFP3 | BPSL1899 | 56 | Type IVB pilus subunit Flp1 (Actinobacillus actinomycetemcomitans; AAN75204) | 46/28 (75) |
| TFP4 | BPSL2752d | 150 | Type IV prepilin leader protein PilE (Pseudomonas aeruginosa; AAA79363) | 46/26 (157) |
| BPSL2756d | 186 | Type IV prepilin leader protein FimT (Pseudomonas aeruginosa; AAB39270) | 38/24 (189) | |
| TFP5 | BPSL3008e | 419 | Type IV pilus biogenesis protein PilB (Pseudomonas aeruginosa; A35384) | 47/34 (569) |
| TFP6 | BPSL3170e | 442 | Type IV pilus biogenesis protein PilQ (Pseudomonas aeruginosa; S37345) | 36/22 (714) |
| Chromosome 2 | ||||
| TFP7 | BPSS1593 | 557 | Type IVB minor pilus subunit PilV (Escherichia coli; AAL05526) | 33/21 (561) |
| BPSS1595 | 184 | Type IVB major pilus subunit PilS (Escherichia coli; BAA77979) | 43/24 (206) | |
| TFP8 | BPSS2185 | 56 | Type IVB pilus subunit Flp1 (Actinobacillus actinomycetemcomitans; AAN75204) | 43/22 (75) |
| BPSS2186 | 72 | Type IVB pilus subunit Flp1 (Actinobacillus actinomycetemcomitans; AAN75204) | 48/28 (82) |
a
Number of amino acids in the predicted protein.
b
Homologues were assigned based on TBLASTN (24) searches in Artemis release 4 (31). Homologues reported are those that have been functionally characterized and published.
c
Number of residues including gaps in the full-length protein alignment.
d
Predicted peptides contain prepilin-like leader sequences.
e
ORF corresponds to the first gene in the predicted locus, as no subunit is present.