TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| B. pseudomallei strains investigated for conservation of pilA by PCR and sequencinga | ||
| 576 (AY598724) | Clinical isolate, Thailand | PHLb |
| 2889 (AY598726) | Pneumonic clinical isolate, Thailand | PHLb |
| NCTC4845 (AY598725) | Monkey isolate | NCTCc |
| B. pseudomallei strains investigated for conservation of pilA by PCR | ||
| 08 | Clinical isolate | 4 |
| GCH | Clinical isolate | Gold Coast Hospitald |
| RBH | Clinical isolate | 4 |
| 06 | Environmental isolate | 4 |
| GF1 | Environmental isolate | 4 |
| GF2 | Environmental isolate | 4 |
| THP375 | Environmental isolate | 4 |
| B. pseudomallei | ||
| K96243 | Clinical isolate, Genr Strr Chls, Thailand | Siriraj Hospitale |
| JAB16.1x | K96243 derivative; merodiploid strain; ΔpilA (nucleotides 67-612)::pAEH16 (cat sacBR oriT oriR6K); Genr Strr Chlr | This work |
| JAB16 | K96243 derivative; unmarked deletion mutant strain; ΔpilA (nucleotides 67-612); Genr Strr Chls | This work |
| E. coli | ||
| OP50 | Uracil auxotroph | 3 |
| S17.1 (λpir) | RP4-2-Tc::Mu Km::Tn7 Tp Sm (λpir) phoA20 thi-1 rpsE rpoB | 28 |
| JABEC16 | S17.1 (λpir) containing pAEH16 | This work |
| Plasmids | ||
| pDM4 | Suicide vector, sacBR oriT oriR6K Chlr | 25 |
| pAEH16 | pDM4 containing the pilA deletion construct | This work |
a
Numbers in parentheses are GenBank accession numbers.
b
Supplied by Ty Pitt, Central Public Health Laboratory, Colindale, United Kingdom.
c
National Collection of Type Cultures.
d
Supplied by D. Alfredson, Gold Coast Hospital, Gold Coast, Australia.
e
Supplied by S. Songsivilai, Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.