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Effect of p38 MAP kinase inhibitor on F. nucleatum-induced collagenase 3 expression in epithelial cells. HaCaT cells were preincubated for 30 min with 10 μM SB203580 (inhibitor of p38) and then cultured in the presence of F. nucleatum (ATCC 25586; 160 bacteria per cell) for 24 h. Culture medium was analyzed by Western blotting, using anti-collagenase 3 antibodies, and RNA extracted from the cells (20 μg) was analyzed for collagenase 3 mRNA levels by Northern blotting.
