TABLE 2.
Protein kinase profile of epithelial cells treated with F. nucleatuma
| Protein name | Abbreviation | Important signaling function | Mol wt (103) | Relative protein levelb (fold increase)
|
|
|---|---|---|---|---|---|
| Control | F. nucleatum | ||||
| Protein kinase C epsilon | PKCe | Apoptosis (10, 60) | 85 | 169 | 343 (2.0) |
| Cyclin-dependent kinase 7 | CDK7 | Cell division (59) | 36 | 528 | 799 (1.5) |
| Cyclin-dependent kinase 9 | CDK9 | Cell division (59) | 35 | 1,158 | 1,855 (1.6) |
| DNA-activated protein kinase | DNA-PK | DNA repair (15) | 211 | 176 | 1,312 (7.4) |
| Casein kinase 2 (37) | CK2a | Cell survival (42, 43) | 38 | 764 | 1,554 (2.0) |
| Casein kinase 2 (35) | CK2a | Cell survival (42, 43) | 35 | 379 | 1,145 (3.0) |
| Death-associated protein kinase 1 | DAPK | Apoptosis (62) | 148 | 2,151 | 4,693 (2.2) |
| Etk/Bmx kinase | Bmx | Cell survival, migration, signal integration (1, 8, 55) | 64 | 376 | 581 (1.5) |
| S6 kinase p70 | S6k | Cell migration (5, 22) | 55 | 326 | 534 (1.7) |
| RhoA kinase | ROKa | Cell migration (23, 56, 69) | 140 | 50 | 117 (2.3) |
| Germinal center kinase | GCK | Stress response (40) | 78 | 706 | 361 (−2.0) |
| G protein-coupled receptor kinase 2 | GRK-2 | Cell proliferation, signal integration (2) | 74 | 864 | 231 (−3.7) |
a
Semiconfluent HaCaT cells were cultured in Dulbecco's modified Eagle's medium the absence of serum for 24 h. A suspension of F. nucleatum (ATCC 25586; 260 bacteria per cell) was then added to the culture medium for 2 h Cells were then collected, washed, and extracted for Kinetworks protein kinase profiling, involving Western blotting for 78 kinases.
b
The values are averages of areas of scanned bands of the blots normalized to correct for differences in the protein content of the samples. The treated samples varied 2 to 28% from each other. Only values showing at least a 50% difference between the control and both of the treated cultures are shown.