TABLE 1.
Strains and plasmid used in this study
| Strain or plasmid | Relevant genotype and/or phenotype | Source or reference |
|---|---|---|
| Strains | ||
| S. aureus | ||
| RN4220 | Restriction-deficient derivative of NCTC 8325-4 | 33 |
| NCTC 8325 | Wild-type strain, rsbU mutant | 21; The Staphylococcal Genetic Stock Center |
| Newman | ATCC 25904; high level of clumping factor, σB+ | 14; laboratory collection |
| ALC638 | agr sarA mutant in Newman background | A. L. Cheung |
| E. coli | ||
| OP50 | Uracil auxotrophy | 6; laboratory collection |
| DH5α | Invitrogen | |
| C. elegans | ||
| Bristol N2 | Wild-type strain | Caenorhabditis Genetics Center |
| Plasmid | ||
| pLTV1 | Temperature-sensitive plasmid harboring Tn917 transposon | 7 |