TABLE 3.
Attenuated S. aureus mutants identified by screening in C. elegans
| Mutants and mutationa | N315 ORFb | Gene annotation | Gene name/function |
|---|---|---|---|
| TCA cycle genes | |||
| 3E1, 4D8, 8D9, 10B10, 22A5, 28C12, 28C11, 29E1 | SA1245 | odhAc,d,e | 2-Oxoglutarate dehydrogenase |
| 25G6, 29B8, 29G6 | SA1244 | odhBc,d,e | Dihydrolipoamide succinyltransferase |
| 7G12 | SA1669 | citG | Fumarate hydratase, class II |
| Transporters | |||
| 5F1 | SA1239 | 5′ braBc,d | Branched chain amino acid transporter |
| 7A2 | SA1214 | opp-2Cc,d,e | Oligopeptide transporter membrane permease |
| Nucleic acid metabolism/DNA replication | |||
| 30A5 | SA1045 | pyrAAe | Carbamoyl-phosphate synthase small chain (pyrimidine/arginine synthesis) |
| 3H1 | SA1288 | dinGc,d,e | DNA helicase |
| 28G12 | SA0467 | 5′ yacA/tilS | Lysidine biosynthesis (isoleucine tRNA synthesis) |
| Miscellaneous | |||
| 29C3 | SA2304 | 5′ fbp | Fructose-bisphosphatase |
| 6A5 | SA0790 | nagD | Conserved hypothetical protein |
| Mutants with no virulence-related phenotype after transduction | |||
| 15G12 | SA1241 | Similar to nitrogen oxide reductase | |
| 31B11 | 3′ SA1239 | 3′ braB | Downstream of braB and SA1238 |
a
Insertional mutants in boldface were transduced into 8325, and their C. elegans-killing phenotypes were confirmed.
b
ORF numbers from the S. aureus N315 genome sequencing project (25).
c
Previously identified in the STM screen of Mei et al. (31).
d
Previously identified in the STM screen of Coulter et al. (11).
e
Upregulated by agr by transcriptional profiling (13).