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. 2017 Jun 9;8(6):1689–1700. doi: 10.1016/j.stemcr.2017.05.004

Figure 5.

Figure 5

Long-Term hESC-Derived Pancreatic Endoderm Cell Grafts Transplanted into the DL Space Contain Glucose-Regulatory Cells

(A) Representative images of Masson's trichrome staining of a PEC graft cross-section in DL site >175 days post transplant, surrounded by collagen (blue) and smooth muscle and erythrocytes (red). Right panel: higher magnification of the inset. Arrows indicate blood vessels. Scale bars represent 100 μm.

(B and C) Representative immunofluorescent staining of PECs engrafted in the DL site harvested after >175 days. Grafts were stained with insulin (red), glucagon (green) (B), somatostatin (green) (C), and nuclear DAPI staining (blue). Right panel: higher magnification of the inset. Scale bars represent 100 μm.

(D) Area of insulin (INS), glucagon (GLU), and somatostatin (SOM) immunoreactivity relative to the total graft area (DAPI+).

(E) Areas of insulin+ relative to glucagon+ or somatostatin+ area for each graft.

∗∗p < 0.05, ∗∗∗p < 0.01, one-way ANOVA with Tukey test for multiple comparisons. All data are presented as mean ± SEM (n = 6 animals [grafts]/2–4 sections per graft).