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. 2017 Jun 9;8(6):1689–1700. doi: 10.1016/j.stemcr.2017.05.004

Figure 6.

Figure 6

Immunohistochemistry of Representative Human Islet Graft Transplanted under the Kidney Capsule of an Immunodeficient Mouse

(A) Representative Masson's trichrome staining of human islet graft cross-section 100 days post transplant at 20×, surrounded by collagen (blue), and smooth muscle and erythrocytes (red). Scale bars represent 100 μm.

(B and C) Fluorescent image of human islet graft staining positive for insulin (red), glucagon (green) (B), somatostatin (green) (C), and nuclear DAPI staining (blue), Scale bars represent 100 μm.

(D) Area of insulin (INS), glucagon (GLU), and somatostatin (SOM) immunoreactivity relative to the total graft area (DAPI+).

(E) Areas of insulin+ relative to glucagon+ or somatostatin+ area for each graft.

∗∗p < 0.01, ∗∗∗p < 0.001; one-way ANOVA with Tukey test for multiple comparisons. All data are presented as mean ± SEM (n = 3 animals [grafts]/2–4 sections per graft).