Fig. 4.

FG-3019 reduces alpha smooth muscle actin (αSMA)-positive cell numbers. a Skin sections were stained with anti-αSMA antibody. Representative images are shown. The αSMA-positive cells were counted in five random high-power fields using a light microscope. The mean score was used for analysis. Each graph represents mean ± SD. b Skin sections were stained with anti-platelet-derived growth factor receptor β (PDGFRβ) antibody. Representative images are shown. c Immunofluorescent staining was performed on cryosections using procollagen antibody. Representative images are shown. Scale bar 50 μm; n = 4 per group; *P < 0.05. DAPI 4′,6-diamidino-2-phenylindole