TABLE 1.
Region | Mean OD450 for antibody toa:
|
|||||
---|---|---|---|---|---|---|
MSP6a | MSP6b | MSP6c | MSP6d | MSP6e | MSP6f | |
Specificity | ||||||
MSP6a | 0.118 | 0.009 | 0.008 | 0.008 | 0.009 | 0.008 |
MSP6b | 0.007 | 0.111 | 0.007 | 0.008 | 0.007 | 0.009 |
MSP6c | 0.009 | 0.008 | 0.084 | 0.018 | 0.006 | 0.007 |
MSP6d | 0.010 | 0.007 | 0.008 | 0.116 | 0.007 | 0.009 |
MSP6e | 0.009 | 0.009 | 0.009 | 0.008 | 0.085 | 0.008 |
MSP6f | 0.007 | 0.008 | 0.007 | 0.008 | 0.009 | 0.092 |
Cross-reactivity | ||||||
MSP3a | 0.007 | 0.007 | 0.008 | 0.010 | 0.007 | 0.012 |
MSP3b | 0.010 | 0.105 | 0.008 | 0.008 | 0.009 | 0.007 |
MSP3c | 0.008 | 0.009 | 0.010 | 0.008 | 0.008 | 0.009 |
MSP3d | 0.010 | 0.009 | 0.007 | 0.049 | 0.008 | 0.008 |
MSP3e | 0.008 | 0.008 | 0.009 | 0.009 | 0.045 | 0.010 |
MSP3f | 0.009 | 0.009 | 0.011 | 0.009 | 0.008 | 0.085 |
Antibodies affinity purified against different regions of MSP6 were tested for specificity and cross-reactivity to related regions in MSP3. Mean OD450s from duplicate wells are shown. All of the peptides were used under identical coating conditions. Boldface values represent positive reactivity.