Figure 1.

A Transcriptional Spectrum of Pluripotent States

(A) ESC culture conditions assayed in this study.

(B) Heatmap showing expression of key pluripotency genes by RNA-seq.

(C) Global transcriptional correlation between all genetic backgrounds and culture parameters.

(D) Principal-component analysis (PCA) showing the relationship between pluripotent ESC states.

(E) Loadings for PCA from (D) showing representative highly weighted genes that drive separation along each component. The histogram shows the distribution of all gene weightings.

(F) PCA analysis showing in vivo pluripotent stages and the full ESC spectrum (left) or defined ESC states separated by genetic background (right).

(G) Fluorescence-activated cell sorting (FACS) plot showing the response of Stella-GFP and MERVL-tdTomato (2C) ESCs in 2i/L to serum.