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. 2017 Jun 9;8(6):1645–1658. doi: 10.1016/j.stemcr.2017.05.014

Figure 3.

Figure 3

Distinct Naive ESC Conditions Activate Master Lineage Regulators

(A) FACS analysis showing near-uniform activation of the ΔPE Oct4-GFP naive pluripotency reporter in ESCs under defined conditions.

(B) Heatmap of differentially expressed genes in pairwise comparisons between naive ESC states. Selected genes from pathways enriched by gene ontology are shown.

(C) Heatmap showing expression of master regulator genes for each of the three primary germ layers and the germline (PGC).

(D) Immunofluorescence staining for master regulators of the germline (BLIMP1), endoderm (SOX17), and neuroectoderm (PAX6) in defined ESC conditions. Scale bars, 50 μm.

(E) FACS plots showing Sox1-GFP activation upon neural induction from indicated ESC conditions.

(F) qRT-PCR showing expression of indicated genes in ΔPE Oct4-GFP ESCs and upon induction of epiblast-like cells (EpiLCs) for 2 days. n = 3 biological replicates each assayed in technical triplicate; error bars, SEM.

(G) Representative examples of primordial germ cell (PGC)-like cell (PGCLC) induction from ancestral ESC conditions. Shown is the percentage of PGCLCs as determined by FACS. Scale bars, 200 μm.