FIG. 3.

Western blot-based assessment of the presence of Bvgⁱ-phase-specific polypeptides other than BipA in B. pertussis. Whole-cell extracts of the indicated strain of B. pertussis grown under Bvg⁺-phase conditions were separated by SDS-PAGE, transferred to polyvinylidene difluoride, and probed with antiserum S3. In each panel, the arrangement of the lanes is as follows: left lanes, wild-type strain; middle lanes, bvgS-I1-carrying derivative; right lanes, bipA mutant constructed either by insertional activation of bipA (A) or by introduction of an in-frame deletion in bipA (B). The positions of molecular size markers are indicated on the left (in kilodaltons).