Table 5.
Mean biodegradation values of acenaphthene and fluorene recorded at flask scale after 14 days.
| Flasks | Temperature (°C) | Initial concentration (ppm) | Residual concentration (ppm) | SD (±) | Percentage degradation (%) |
|---|---|---|---|---|---|
| A (Ace + R. ornithinolytica) | 25 | 50 | 22.38 | 0.54 | 55.2 |
| 30 | 50 | 3.72 | 0.8 | 92.6 | |
| 35 | 50 | 2.31 | 1.5 | 95.4 | |
| 37 | 50 | 1.09 | 0.2 | 97.8 | |
| 38 | 50 | 0.21 | 0.3 | 95.8 | |
| 40 | 50 | 13.41 | 1.23 | 73.2 | |
| 45 | 50 | 18.56 | 0.9 | 62.9 | |
| B (Ace + S. marcescens) | 25 | 50 | 27.49 | 0.02 | 45 |
| 30 | 50 | 22.38 | 0.89 | 55.2 | |
| 35 | 50 | 11.33 | 0.96 | 77.3 | |
| 37 | 50 | 4 | 1.63 | 91.9 | |
| 38 | 50 | 4.14 | 0.64 | 91.7 | |
| 40 | 50 | 12.29 | 0.45 | 75.4 | |
| 45 | 50 | 13.16 | 1.19 | 73.7 | |
| C (Ace + B. megaterium) | 25 | 50 | 17.58 | 0.63 | 64.8 |
| 30 | 50 | 5.82 | 0.32 | 88.4 | |
| 35 | 50 | 13.41 | 0.74 | 73.2 | |
| 37 | 50 | 18.62 | 1.19 | 62.8 | |
| 38 | 50 | 18.57 | 1.6 | 62.8 | |
| 40 | 50 | 19.25 | 3.56 | 61.5 | |
| 45 | 50 | 19.5 | 3.36 | 60.9 | |
| D (Ace + A. hydrophila) | 25 | 50 | 12.29 | 3.98 | 75.4 |
| 30 | 50 | 0.28 | 0.23 | 99.4 | |
| 35 | 50 | 0.24 | 0.06 | 99.5 | |
| 37 | 50 | 0.42 | 1.75 | 99.2 | |
| 38 | 50 | 5.06 | 1.97 | 89.88 | |
| 40 | 50 | 18.62 | 2.67 | 62.8 | |
| 45 | 50 | 22.38 | 0.35 | 55.2 | |
| (Ace + cocktail) | 25 | 50 | 13.1 | 0.03 | 73.8 |
| 30 | 50 | 0.28 | 0.65 | 99.4 | |
| 35 | 50 | 0.3 | 0.54 | 99.4 | |
| 37 | 50 | 0.42 | 0.34 | 99.2 | |
| 38 | 50 | 0.02 | 0.74 | 99.9 | |
| 40 | 50 | 0.42 | 0.56 | 91.5 | |
| 45 | 50 | 4.78 | 0.21 | 90.4 | |
| J (fluorene + R. ornithinolytica) | 25 | 50 | 4.22 | 1.23 | 91.6 |
| 30 | 50 | 1.96 | 1.12 | 96.1 | |
| 35 | 50 | 0.75 | 1.69 | 98.5 | |
| 37 | 50 | 0.02 | 0.65 | 99.9 | |
| 38 | 50 | 0.18 | 0.36 | 99.6 | |
| 40 | 50 | 4.22 | 0.39 | 91.6 | |
| 45 | 50 | 4.33 | 0.98 | 91.6 | |
| K (fluorene + S. marcescens) | 25 | 50 | 18.87 | 0.36 | 62.3 |
| 30 | 50 | 3.65 | 0.21 | 92.3 | |
| 35 | 50 | 2.28 | 0.14 | 95.4 | |
| 37 | 50 | 1.06 | 0.47 | 97.9 | |
| 38 | 50 | 1.07 | 0.13 | 97.7 | |
| 40 | 50 | 13.29 | 0.36 | 73.4 | |
| 45 | 50 | 38.4 | 0.97 | 23.2 | |
| L (fluorene + B. megaterium) | 25 | 50 | 2.68 | 1.16 | 94.6 |
| 30 | 50 | 2.28 | 1.23 | 95.4 | |
| 35 | 50 | 3.85 | 4.56 | 92.3 | |
| 37 | 50 | 3.87 | 4.41 | 92.3 | |
| 38 | 50 | 3.87 | 2.13 | 92.3 | |
| 40 | 50 | 14.2 | 0.12 | 71.4 | |
| 45 | 50 | 3.08 | 0.96 | 93.8 | |
| M (fluorene +A. hydrophila) | 25 | 50 | 12.7 | 1.1 | 74.4 |
| 30 | 50 | 2.39 | 0.36 | 95.2 | |
| 35 | 50 | 0.44 | 0.21 | 99.1 | |
| 37 | 50 | 0.73 | 0.15 | 98.5 | |
| 38 | 50 | 12.79 | 0.92 | 74.4 | |
| 40 | 50 | 14.71 | 0.89 | 64.6 | |
| 45 | 50 | 21.36 | 6.39 | 57.3 | |
| N (fluorene + cocktail) | 25 | 50 | 0.69 | 1.69 | 98.6 |
| 30 | 50 | 0.25 | 2.36 | 99.5 | |
| 35 | 50 | 0.31 | 3.35 | 99.4 | |
| 37 | 50 | 0.29 | 2.13 | 99.4 | |
| 38 | 50 | 0.3 | 0.36 | 99.4 | |
| 40 | 50 | 0.25 | 0.36 | 99.5 | |
| 45 | 50 | 0.25 | 0.38 | 99.5 |
Key: Ace: acenaphthene, Cocktail: R. ornithinolytica, S. marcescens, B. megaterium, A. hydrophila.