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CREB regulates miR-20b expression. RAW 264.7 cells were transfected with either siRNA control or CREB siRNA for 48 h. To detect miR-20b levels, qRT-PCR was used (A) and the relative expression of miR-20b to U6 snRNA was calculated (B). The expression of CREB mRNA and protein were detected by qPCR (C) and Western blot (D) analyses. Data are expressed as the mean ± standard deviation of four independent experiments; *p < 0.05, **p < 0.01 compared with the Control group
