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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Immunol. 2017 Apr 21;198(11):4293–4303. doi: 10.4049/jimmunol.1700261

Figure 4. Ent protects against neutrophil-mediated bacterial killing.

Figure 4

(A) Neutrophil-mediated bacteria killing assay in presence of exogenous Ent (25 μM) with WT E. coli. (B) PMNs-mediated bacteria killing assay in presence of exogenous Ent (25 μM) with WT E. coli. (C) WT E. coli and its isogenic mutants employed in the killing assay to assess the effect of endogenously-derived Ent. (D and E) Phagocytosis (%) was determined by flow cytometric analysis of opsonized GFP-expressing E. coli by neutrophils. In vitro assays were performed in triplicates and are representative of two independent mouse experiments. Results are expressed as mean ± SEM. * p< 0.05, ** p<0.01, and *** p<0.001.