Figure 6. The Differential Impact of Autophagy Inhibition on Long-Lived and Short-Lived Proteins.

(A) The average Δk_deg for subsets of proteins with varying degradation rates in ATG5^−/− and ATG7^−/− cells. The trend is consistent with the idea that the change in degradation rates of long-lived proteins is primarily due to reduced flux through the autophagy pathway whereas short-lived proteins may be de-stabilized due to increased proteasome levels. Bars indicate the mean of Δk_deg measurements for subsets of proteins with the indicated range of k_deg values, and error bars indicate SEM.

(B) Steady-state SILAC analysis indicates that, as a group, relative expression levels of long-lived proteins are increased and relative expression levels of short-lived proteins are decreased in ATG5^−/− and ATG7^−/− cells. Bars indicate the mean of the log₂ ratio of expression levels between ATG5^−/− and WT^+vector cells for subsets of proteins with the indicated range of k_deg values, and error bars indicate SEM.