Fig 1. LCL521 represents an acute and potent inhibitor of ACDase.

(A) MCF7 cells were treated with vehicle, or with 0.1, 0.25, 0.5, 1, 1.5, 2.5, 5, and 10μM LCL521 for 1h. Cer, Sph and S1P were then extracted and quantified by LC-MS/MS. (n = 4, three times experiments with one time duplicates and two times single experiment). The actual amounts of Cer, Sph and S1P after treatment with LCL521 are presented in S1B–S1D Fig (B). MCF7 cells were treated with vehicle or 1μM LCL521 for 15min, 30min, 1, 2, and 5h. Sph was then extracted and quantified by LC-MS/MS. (*p<0.05, n = 7, 3 times experiments with 2 times duplicates and 1 time triplicates).