Fig 2. Synergistic effect of LCL521 and single dose of ionizing radiation.

MCF7 cells were irradiated with 0 or 2Gy using 137 Cesium irradiator. 1h after IR, each replicated treatment were further treated with vehicle or 5x 1μM LCL521. For the 5-time treatment, media were replaced every 24h with fresh media that contained either vehicle or 1μM LCL521. After that, cells were cultured for 4 weeks and then stained with crystal violet (1g/500ml formalin). Representative crystal staining is shown. Colonies count is presented in S2 Fig.