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. 2017 Jun 14;12(6):e0178746. doi: 10.1371/journal.pone.0178746

Fig 1. TRAIL expression in NDV-stimulated NK cells of mice where IFN-γ receptor one was deficient.

Fig 1

(A) Purified splenic NK cells from IFN-R1-/- mice were stimulated with NDV 7793 or IFN-γ. Cells were stained with a PE-conjugated monoclonal antibody (mAb) against TRAIL and then examined by flow cytometry. PBS-treatment NK cells were used as negative controls. Results are representative of three independent experiments. (B) Purified splenic NK cells from IFN-R1+/+ and IFN-R1-/- mice were stimulated with NDV 7793 or IFN-γ. Cellular proteins were extracted and subjected to western blotting using a mAb against TRAIL. Results are representative of three independent experiments. (C) The concentration of IFN-γ in supernatants was determined by ELISA. Values represent the mean + standard deviation from triplicate wells, with results obtained from three independent experiments. *P<0.01 and ΔP<0.01 vs. unstimulated NK cells in IFN-R1+/+ and IFN-R1-/- mice, respectively.