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. 2017 Jun 14;12(6):e0178746. doi: 10.1371/journal.pone.0178746

Fig 2. TRAIL expression in IFN-R1-/- NK cells following stimulation with NDV-HN.

Fig 2

(A) Purified murine splenic IFN-R1-/- NK cells were incubated with NDV 7793, recombinant HN protein, or F protein, respectively. Cells were stained with PE-conjugated anti-TRAIL mAbs. Unstimulated NK cells were used as negative controls. (B) Total cellular protein was extracted and subjected to western blotting using a mAb against TRAIL; β-actin was used as an internal loading control. Results are representative of three independent experiments.