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. 2017 Jun 14;12(6):e0178746. doi: 10.1371/journal.pone.0178746

Fig 3. Recombinant NDV HN upregulated TRAIL expression in NK cells by binding to NKp46.

Fig 3

(A) Purified murine splenic IFN-R1-/- NK cells were exposed to HN, NDV 7793, or a mixture of NDV 7793 and neutralizing antibodies against HN, and then incubated with a PE-conjugated mAb against NKp46. PBS-treatment NK cells were used as negative controls. (B) TRAIL protein expression in NK cells where NKp46 was recognized by NDV HN protein. Purified IFN-R1-/- NK cells were treated with neuraminidase for desialylation prior to stimulation with HN. Alternatively, IFN-R1-/- NK cells were treated with a mixture of NDV 7793 and neutralizing antibodies against HN. Cellular proteins were extracted and subjected to western blotting using a mAb against TRAIL. Results are representative of three independent experiments.