Production of NO by RAW 264.7 mouse macrophage cells 48 h after LTA-9.5 (black bars), LTA-10.5 (open bars), or LTA-0 (stippled bars) treatment at the indicated doses (in micrograms per milliliter) (A) and production of IL-8 by A549 cells in response to no stimulus (None), LTA-9.5, LTA-10.5, or LTA-0 (B). Error bars indicate standard deviations for the means of three replicate data points. At 50 and 100 μg/ml, the NO production in response to LTA-9.5 was significantly greater than the responses to LTA-10.5 or LTA-0 (P < 0.001 by Student's t test). IL-8 production in response to LTA-9.5 or LTA-10.5 was significantly greater (P < 0.001 by Student's t test) than to no stimulus or to LTA-0.