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. 2017 Jun 14;12(6):e0179342. doi: 10.1371/journal.pone.0179342

Fig 6. Analysis of the MMP activity by in situ zymography.

Fig 6

Representative cross-section images displayed a homogenous MMP activity within the vascular tissue of the ROS groups at early stages such as t = 0 (A) as well as at late stages such as t = 56 d (B). In contrast, the ROS group at t = 8 d revealed a pronounced, focal MMP activity at the treated focus (C; brackets), whereas no comparable finding was to be noticed in its control group (D). In situ zymography: MMP activity (green) with DAPI-counterstain (blue); scale bars = 500 μm. (E) Semi-quanitative analysis of the relative MMP activity. ROS = ROS group; C = control group; ** p < 0.01; *** p < 0.001).