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. 2017 May 31;13(5):e1006827. doi: 10.1371/journal.pgen.1006827

Fig 8. Meiotic crossover frequency of I2b in WT and triple heterozygote via flow cytometry.

Fig 8

(A) Pollen grains from plants that are hemizygous for the DsRed2 (red grains) and eYFP (green pollen grains) markers flanking the I2b interval visualized using bright field (BF) microscopy or epi-fluorescence (R+G). Pollen grains expressing both markers are yellow. (B) Side scatter (SSC)/ forward scatter (FSC) plot of eYFP-DsRed/-- pollen grains (R1 gate) in WT with the number of pollen grains counted. Events in the R1 gate are represented as green points. (C) Side scatter (SSC)/ forward scatter (FSC) plot of eYFP-DsRed/-- pollen grains (R1 gate) in triple heterozygote with the number of pollen grains counted. Events in the R1 gate are represented as green points. (D) The R2 gate is comprised of yellow-only pollen grains; the R3 gate is comprised of the yellow+red pollen grains; the R4 gate R4 is comprised of non-fluorescent pollen grains; the R5 gate is comprised of red-only pollen grains. (E) DsRed / eYFP plot of eYFP-DsRed/-- pollen grains in WT with R2-R5 gates with the percentage of events in each quadrant is shown in each gate. (F) DsRed / eYFP plot of eYFP-DsRed/-- pollen grains in triple heterozygote with R2-R5 gates with the percentage of events in each quadrant is shown in each gate. (G) Formula to calculate the genetic distance of an interval flanked by the two fluorescent markers with results in WT and triple heterozygote. Standard deviations are shown in parentheses. Z score is 185.4: P value << 0.01.