Fig 5. In vitro assembled nucleocapsid cores.

(A) Negative stain image of purified rubella virus nucleocapsid cores. Black arrows indicate the co-purified capsid tetrameric units. Scale bar is 500 Å long. Agarose gel analysis of the effect of nuclease on the nucleocapsid cores is shown in S5 Fig. (B) Negative stain images of linear assemblies of capsid tetramers. Scale bar is 100 Å long. In panels A and B, white is high density. (C) and (D) Left panels show tomogram cross-sections from nucleocapsid core particles. Right panels show the end-on view of the nucleocapsid core surface that are indicated in the left panels by a dashed black line. Scale bars in panel C and D are 50 Å and 100 Å long, respectively. Black represents high density. (E) Side view of the sub-tomogram averaged density of the recombinantly produced nucleocapsid tetramers. (F) A single capsid unit density showing the fitted C-terminal domain of the capsid protein. Left panel shows the side view whereas the right panel shows the top view. Scale bar is 25 Å long. A more detailed fitting result for panel F is given in S6 Fig. See also S1 Table.