FIG. 7.

Intracellular replication of the lpp-msbB TKO mutant inside T84 cells (A) and RAW264.7 cells (B). Cells were infected with the various S. enterica serovar Typhimurium mutants and WT S. enterica serovar Typhimurium for 1 h. The cells were washed and incubated for 1 h with gentamicin (100 μg/ml)-containing medium. After incubation, the cells were washed and incubated in fresh medium containing a minimum concentration of gentamicin (5 μg/ml) for different times (1, 6, 12, and 24 h) (46). Finally, the cells were lysed with 0.1% Triton X-100 and plated on MSB agar plates to determine the number of CFU. Three independent experiments were performed; data from a representative experiment are shown.