Table 1.

Histology, IHC, and Genetic Analysis of Adrenal Tumors and Adjacent Normal Adrenal Tissue

	Size (mm)	ZF-Like Cell (Area %)	ZG-Like Cell (Area %)	ZR-Like Cell (Area %)	Histological Diagnosis	CYP11B2	CYP17A	Somatic Mutation
T1	21 × 13	85	15	0	Adenoma	Heterogeneously positive	(−)	KCNJ5	L168R
T2	12 × 4	0	100	0	Adenoma	Homogeneously positive	Weak positive	CACNA1D	G403R
T3	17 × 11	0	0	100	Adenoma	Positive in 10% of the cells	Weak positive	N/D	N/D
T4	7 × 1	90	10	0	Adenoma	50% of ZF-like cells positive	(−)	KCNJ5	T158A
T5	4 × 3	80	20	0	Adenoma	(−)	(−)	N/D	N/D
N1					Normal			N/D	N/D

The results of histology, IHC, and targeted next-generation sequencing are shown by each component. The sample numbers match those in Fig. 1(a) and 1(d). Two different KCNJ5 somatic mutations were each identified in two APAs predominantly consisting of ZF-like cells (T1, T4), whereas CACNA1D somatic mutation was detected in the APA predominantly consisting of ZG-like cells (T2). No somatic mutations were detected in the APA with ZR-like cells (T4), the APA without CYP11B2 IHC (T5), and the adjacent normal adrenal tissue (N1). Reference transcript sequences used for determining amino acid changes are as follows: NM_000890.3 (KCNJ5), NM_001128839.2 (CACNA1D), NM_021949.3 (ATP2B3), and NM_000701.7 (ATP1A1).

Abbreviation: N/D, not detected.