Skip to main content
. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Immunol. 2017 May 3;198(11):4352–4359. doi: 10.4049/jimmunol.1601792

Figure 6.

Figure 6

The relative roles of IL-2 and IL-4 signaling in type II cytokine production. Polarized Th2 cells were stimulated with PMA and ionomycin in the presence or absence of monensin, IL-2/CD25 blocking antibodies, IL-4 blocking antibodies or both IL-2/25 and IL-4 blocking antibodies. Cells were harvested 4 hours after stimulation for pSTAT5 and pSTAT6 analysis (A) and 6 hours after stimulation for analysis of cytokine gene expression (B). These data represent 3 independent experiments with cultures derived from 3 mice per experiment. *p<0.05, significantly decreased as compared to PI-stimulated controls. #p<0.05, significantly decreased from cells stimulated in the presence of IL-2/CD25 blocking antibodies.