Author response image 1.
(A) PINCR-WT HCT116 cells were transfected with CTL siRNA (siCTL) or p21 siRNAs (sip21) for 48 hr and p21 knockdown was measured by immunoblotting. GAPDH was used as loading control. (B) PINCRWT HCT116 cells were transfected for 48 hr with siCTL or sip21 or co-transfected with sip21 and siCTL or siBTG2 or siGPX1 or siRRM2B. PI-staining and FACS analysis was performed after 48 hr of 5-FU treatment. Error bars represent SD from three biological replicates. *p<0.05; #p<0.01; **p<0.005; ##p<0.001.