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. 2017 Jun 15;2(12):e92295. doi: 10.1172/jci.insight.92295

Figure 1. α-Synuclein protein is expressed in STC-1 cells.

Figure 1

(A) Relative quantitation of Gapdh, α-synuclein (Snca), cholecystokinin (Cck), and peptide YY (Pyy) mRNAs in STC-1 and SH-SY5Y cells showing that α-synuclein mRNA is present in STC-1 cells. β-Actin (Actb) was used as the normalizer, and RNA isolated from HeLa cells served as a comparator. Data represent mean ± SEM of 3 individual experiments. (B) Coomassie Blue–stained 4%–12% gradient SDS-PAGE gel and corresponding immunoblot of STC-1 cells, A53T mouse brain, and Snca-/- mouse brain extracts with α-synuclein monoclonal antibody. A nonspecific band (asterisk) was detected in A53T and Snca-/- mouse brain extracts. The molecular weights of protein bands present in the ladder are indicated in kDa. (C) 3D Z-stack image of STC-1 cells showing α-synuclein (red) in the cytoplasm. Cytoskeletal staining is shown with β-tubulin (green). Scale bar: 20 μm.