Serial dilutions of plasma samples were analyzed for their binding (A) to E glycoprotein of ZIKV (GZ01, KU820898), DENV1 (GZ35, KP723476), and DENV2 (GZ05, KP012546) by ELISA and for neutralizing activity (B) against ZIKV (GZ01), DENV1 (Hawaii strain), and DENV2 (New Guinea strain) by plaque assay. All results were derived from at least 2 independent experiments.