Figure 1. CD146 and CCR5 expression within CD4⁺ Tcon (non CD25^hiCD127^low within CD4⁺ T cells) and Treg (CD25^hiFoxP3⁺ within CD4⁺ T cells) subsets.

(A) Color plots representing the gating strategy of CD146-expressing cells according to CD45RA, and representative examples of CD146⁺ and CD146⁺CCR5⁺ subsets (in CD4⁺ T live lymphocyte gate) in 1 active cGvHD patient and 1 patient without cGvHD. (B–E) Data shown are dot plots (mean ± SEM) according to different clinical groups: No (n = 20) or active cGvHD (n = 20) and different clinical grades of cGvHD according to NIH criteria (mild, n = 8; moderate, n = 8; severe, n = 4). Unpaired t test comparing the frequencies between groups. Comparisons in B–E were analyzed using the Wilcoxon rank-sum test.