(A) Percentage of new Tregs in the spleen for each cohort at day +14 and +28, following 7 days of labeling. Data for 5 independent experiments pooled for day +7 to +14 analysis, and 3 independent experiments for day +21 to +28 analysis. (B) Percentage of new Tregs in the liver for each cohort measured at day +14 and +28, following 7 days of labeling. Data for 3 independent experiments were pooled for day +7 to +14 analysis and 2 independent experiments for day +21 to +28 analysis. (C) Percentage of new Treg and Tem cells in the allogeneic spleen measured at day +14 and +28, following 7 days of labeling. Data for 5 independent experiments pooled for the day +7 to +14 analysis, and 3 independent experiments for day +21 to +28 analysis. (D) Percentage of new Treg and Tem cells in the allogeneic liver measured at day +14 and +28, following 7 days of labeling. Data for 3 independent experiments pooled for day +7 to +14 analysis, and 2 independent experiments for day +21 to +28 analysis. For panels A and C, n = 2–7 mice; for panels B and D, n = 5-8 mice; **P < 0.01; Tukey’s test with 2-way ANOVA.