Figure 5. DNA-PKcs knockdown attenuates progerin-induced inhibition of VSMC growth.

A. VSMCs were infected with a retroviral vector encoding progerin with or without siRNA targeting DNA-PKcs, after which expression of DNA-PKcs was examined by western blot analysis. B. Population doubling was examined using VSMCs prepared as explained in Figure 5A. In some groups, VSMCs were infected with a retroviral vector encoding progerin with or without siRNA targeting p53, after which population doubling was examined. C. Representative pictures of VSMCs prepared as explained in (A). Scale bar = 100 μm.