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. 2017 Mar 15;8(21):34099–34110. doi: 10.18632/oncotarget.16212

Figure 1. LPS induced the expression of Tec and phosphorylation of Tec protein.

Figure 1

A. The expression of Tec was detected by western blotting after LPS stimulus at different concentration (0.01, 0.1, 1, 10, 100 μg/ml). B. Quantitative analysis of Tec protein was determined by integral optical density (*P < 0.05, **P < 0.01, n = 3.). C. The expression of Tec and p-Tec was detected by Western blotting after 0.1 μg/ml LPS stimulus. Total Tec protein was immunoblotted with an anti-Tec Ab, and tyrosine phosphorylation was detected by immunoprecipitating with an anti-phosphotyrosine Ab. D.-E. The relative levels of Tec and p-Tec were quantified. 0.1 μg/ml LPS can upregulate the expression of Tec and p-Tec with a peak at 60 min. (*P < 0.05, **P < 0.01, n = 3.).