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. 2017 Mar 3;8(21):34164–34176. doi: 10.18632/oncotarget.15878

Figure 5. PVT1 regulates LASP1 expression by acting as molecular sponge for miR-203.

Figure 5

A. Schematic representation of the predicted target site of miR-203 in LASP1 3'-UTR. B. qPCR analysis of miR-203 expression after transfection with the miR-203 mimic and miR-101 inhibitor in Eca109 and KYSE150 cells. C. Luciferase reporter assay for LASP1 mRNA 3'-UTR following miR-203 ectopic expression. D. Relative LASP1 mRNA level after transfected with control (NC), miR-203 mimic or inhibitor. E. Relative LASP1 mRNA level after knockdown of PVT1, or transfected with miR-203 inhibitor, or with the pcDNA-LASP1 lacking the 3'-UTR in Eca109 and KYSE150 cells. F. Western blot of LASP1 protein level after knockdown of PVT1, or transfected with miR-203 inhibitor, or with the pcDNA-LASP1 lacking the 3'-UTR in Eca109 and KYSE150 cells. Error bars indicate means ± S.E.M. *P < 0.05.