TABLE 1.
S. pneumoniae strain EF3030 in tissuea
| Day post Challenge | Mean level of S. pneumoniae strain EF3030 (102 CFU) (± SEM)
|
|||
|---|---|---|---|---|
| Spleen | Cervical lymph nodes | Lung | Nasal tract | |
| 1 | <0.02 | <0.02 | <0.02 | 5.56 (± 0.83) |
| 2 | <0.02 | 0.16 (± 0.02) | 0.78 (± 0.12) | 562.34 (± 74.35) |
| 4 | <0.02 | <0.02 | 0.28 (± 0.08) | 1,318.26 (± 183.58) |
| 7 | <0.02 | <0.02 | 0.12 (± 0.02) | 3.16 (± 0.46) |
| 14 | <0.02 | <0.02 | 0.03 (± 0.02) | 5.01 (± 0.72) |
| 21 | <0.02 | 0.04 (± 0.02) | <0.02 | 39.81 (± 5.87) |
a
BALB/c mice were nasally challenged with ∼7.5 × 106 CFU of S. pneumoniae strain EF3030 in a 15-μl volume of Ringer's solution. Nasal tract-, lung-, cervical lymph node-, and spleen-associated pneumococci were enumerated 1, 2, 4, 7, 14, and 21 days postchallenge and are expressed as mean CFU per mouse for five mice; data are representative of three different experiments. Uninfected (negative control) mice had no detectable S. pneumoniae (i.e., < 0.02 × 102), and these values are not shown.