Table 1. orthotopic recycling of bladder cancer cell lines.
| Cycle | Mice Inoculated (n) | Successful Bladder Grafting (n) | Median progression time to large tumor (1010photon count) (weeks) | Mice with LN Metastasis (n) | Mice with Distant Metastasis (n) |
|---|---|---|---|---|---|
| UM-UC9 | |||||
| 1 | 2 | 2 (100%) | 14 | 0 | 0 |
| 2 | 5 | 4 (80%) | 10 | 0 | 0 |
| 3 | 4 | 4 (100%) | 5 | 1 (25%)* | 0 |
| 4 | 16 | 15 (94%) | 5 | 0 | 0 |
| UM-UC6 | |||||
| 1 | 2 | 2 (100%) | 12 | 0 | 0 |
| 2 | 8 | 8 (100%) | 10 | 0 | 0 |
| 3 | 3 | 3 (100%) | 4 | 0 | 0 |
| 4 | 3 | 3 (100%) | 5 | 0 | 0 |
| UM-UC14 | |||||
| 1 | 3 | 3 (100%) | 11 | 0 | 0 |
| 2 | 3 | 3 (100%) | 8 | 0 | 0 |
| 3 | 2 | 2 (100%) | 3 | 0 | 0 |
| 4 | 16 | 15 (94%) | 3 | 2 (13%)* | 1 (6%)* |
| 5 | 5 | 5 (100%) | 4 | 0 | 0 |
| 6 | 5 | 5 (100%) | 4 | 0 | 0 |
| 7 | 8 | 7 (88%) | 3 | 1 (13%)* | 0 |
| 8 | 20 | 20 (100%) | 3 | 1 (5%)* | 0 |
| UM-UC3 | |||||
| 1 | 5 | 2 (40%) | 10 | 0 | 0 |
| 2 | 5 | 3 (60%) | 9 | 2 (40%)* | 1 (20%)* |
| 3 | 5 | 5 (100%) | 6 | 5 (100%)* | 4 (80%)* |
| 4 | 5 | 4 (80%) | 5 | 4 (80%)* | 4 (80%)* |
| 5 | 3 | 2 (67%) | 3 | 2 (67%)* | 2 (67%)* |
| 6 | 15 | 13 (87%) | 3 | 12 (80%)* | 5 (33%)* |
| 7 | 20 | 20 (100%) | 3 | 18 (90%)* | 9 (45%)* |
| 8 | 18 | 17 (94%) | 4 | 15 (83%) | 10 (56%) |
| UM-UC13 | |||||
| 1 | 5 | 2 (40%) | 16 | 0 | 0 |
| 2 | 5 | 4 (80%) | 9 | 3 (60%)* | 3 (60%)* |
| 3 | 2 | 2 (100%) | 5 | 2 (100%)* | 1 (50%)* |
| 4 | 2 | 2 (100%) | 6 | 2 (100%) | 2 (50%) |
Labeled (luciferase and red fluorescent protein) human BC cell lines were orthotopically recycled according to previously published methodology. Growth became more aggressive with successive generations (cycles). The cell lines UM-UC3 and UM-13 are characteristically mesenchymal, and their propensity for metastasis increased with successive recycling.
*In case of lymph node or distant metastases cell suspension was performed from metastases.