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. 2017 Mar 7;8(21):34268–34282. doi: 10.18632/oncotarget.15965

Figure 8. Impact of piR-35127, piR-46545, anti-piR-34871, and anti-piR-52200 on colony formation.

Figure 8

Normal lung epithelial cells (CRL9482, panel A) and mammary epithelial cells (MCF-10A, panel B) stably over-expressing green fluorescent protein (GFP) were plated in 96-well plates and were transfected with 500 nM of scrambled piRNA (control), piR-35127, piR-46545, anti-piR-34871, or anti-piR-52200. Cells were imaged and photographed 5–7 days post-transfection using a fluorescent microscope. Cell colonies in four microscopic fields per well were counted and the average cell colony number count of control and experimental was plotted. Cells transfected with the four piRNAs formed significantly fewer colonies compared to cells transfected with the scrambled piRNA. The data suggests that over-expression of piR-35127 and piR-46545 and knock-down of piR-34871 and piR-52200 attenuates cell proliferation. All experiments were done at least 3 independent times with n = 4 wells per treatment. The (*) indicates statistical significance compared to controls, with a P < 0.02.