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. 2017 Mar 13;8(21):34362–34373. doi: 10.18632/oncotarget.16156

Figure 3. Notch3 knockdown inhibits cell growth in vitro.

Figure 3

(A) Notch3 levels in T24 and J82 cells after treatment with Notch3-shRNAs (shNotch3-1 and shNotch3-2) or scrambled shRNA (control), determined by western blot. (B) Cell proliferation of T24 and J82 cells after treatment with Notch3-shRNA or scrambled shRNA was assessed by CCK-8 kit, and mean ± SD from three independent experiments. (C) Colony formation efficiency was assessed in T24 and J82 cells after treatment with Notch3-shRNA or scrambled shRNA. Representative photographs from three independent experiments. (D) Expression of Ki-67 levels in T24 cells after treatment with Notch3-shRNA or scrambled shRNA, determined by immunofluorescence. Representative photomicrographs from three independent experiments. Scale bar, 50 μm.