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The expression of γ-H2AX was assessed by western blotting in MM cell lines treated with CUR at 25 μM or with DMSO for 24 and 30 hours. Actin was used as an internal control. The intensity of the bands was quantified using the ImageJ software after blot scanning of two independent experiments. The densitometric ratios between γ-H2AX and actin are reported.
