Figure 6. Effect of CUR on the expression and activation of signaling pathway molecules.

Western blotting analysis was performed on MM cells treated with CUR (25 μM) or DMSO vehicle for 24 hours. The levels of pERK1 and pERK2 proteins, as well as p-p38 protein, pJNK, p-c-Jun and pAKT proteins were compared with that of total ERK, p38, JNK, c-Jun and AKT proteins, respectively. The ratios are reported. Actin and tubulin were used as an internal control. n.d= not detectable.