Table 1. EC50 values for compounds #1 and #6 for pancreatic cancer cell viability.
| Cell Line | Compound #1 (μM) | Compound #6 (μM) |
|---|---|---|
| AsPC-1 | 19.1 ± 2.5 | 14.7 ± 0.9 |
| Capan1 | 21.7 ± 2.4 | 24.2 ± 1.8 |
| CD18/HPAF | 22.5 ± 4.7 | 23.5 ± 2.2 |
Pancreatic cancer cells were seeded in 96-well plates at 3 × 103 cells/well for AsPC-1 and 2 × 103 cells/well for Capan1 and CD18/HPAF in culture medium. Cells were treated with increasing concentrations of compound #1 or #6 (0, 1.6, 3.1, 6.3, 12.5, 25, 50 and 100 μM) and incubated for 7 days at 37°C. Viabilities of the treated cells were determined using PrestoBlue reagent as described in Material and Methods, and the obtained results were normalized to vehicle control. Half-maximal effective concentration (EC50) for the inhibition of clonogenic survival of the indicated cells by each compound was determined using SigmaPlot 11.0 software. The results were shown as mean ± S.D. from two sets of experiments in duplicate samples (n = 4).