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. 2017 Mar 29;8(21):34687–34697. doi: 10.18632/oncotarget.16670

Figure 2. JAK1 S646P mutation induces constitutive activation of JAK-STAT and MAPK-ERK signaling pathways.

Figure 2

(A) Autophosphorylation of JAK1 protein in HEK293V cells transiently transfected with wild-type (WT) or a mutant of JAK1 (A639G, S646P, P960S and V658F). (B) Phosphorylation of STAT3, ERK1/2 and AKT in HEK293V cells. Protein lysates of HEK293V cells transiently transfected with WT JAK1 or indicated mutants were analyzed by Western blot using specific antibodies shown. GAPDH was used as loading control. Similar results were obtained in 3 independent experiments.